Large-scale advances in SSR markers with high-throughput sequencing in Euphorbia fischeriana Steud
Large-scale advances in SSR markers with high-throughput sequencing in Euphorbia fischeriana Steud
Blog Article
Background: Euphorbia fischeriana Steud is a very important medicinal herb and has significant medical value for healing cancer, edema and tuberculosis in China.The lack of molecular markers for Euphorbia fischeriana Steud is a dominant barrier to genetic research.For the purpose of developing many simple sequence repeat (SSR) molecular markers, we completed transcriptome analysis with the Illumina HiSeq 2000 platform.
Results: Approximately 9.1 million clean reads were acquired and then assembled into approximately 186.3 thousand nonredundant unigenes, 53,146 of which were SSR-containing unigenes.
A total of 76,193 SSR loci were identified.Of these SSR loci, 28,491 D-Aspartic Acid were detected at the terminal position of ESTs, which made it difficult to design SSR primers for these SSR-containing sequences, and the residual SSRs were thus used to design primer pairs.Analyzing the results of these markers revealed that the mononucleotide motif A/T (44,067, 57.
83% of all SSRs) was the most abundant, followed by the dinucleotide type AG/CT (9430, 12.38%).Using 100 randomly selected primer pairs, 77 primers were successfully amplified in Euphorbia fischeriana Steud, and 79 were successfully amplified in Ball - Field Equipment - Maintenance three other related species.
The markers developed displayed relatively high quality and cross-species transferability.Conclusions: The large number of EST-SSRs exploited successfully in Euphorbia fischeriana Steud for the first time could provide genetic information for research on linkage maps, variety identification, genetic diversity analysis, and molecular marker-assisted breeding.How to cite: Li H, Ma Y, Pei F, et al.
Large-scale advances in SSR markers with high-throughput sequencing in Euphorbia fischeriana Steud.Electron J Biotechnol 2021;49.https://doi.
org/10.1016/j.ejbt.
2020.11.004.